my nanoparticle is aggregated in dmem during tissue culture how can i overcome this problem?if i can incubate my nano with cells for certain time till uptaking some of my nano without media then washing and add dmem media? or what i can do?...
In plain Media without FBS it agglomerates. so use media with FBS which replaces the capping and bound the particle.Try MEM instead of DMEM. but both works well without agglomeration. Try to change capping material. If it is weak capping agent it will not work.And Also after incubation remove media and don't wash it. I hope it works. All the Best