I have a PCR product with 260bp size , usually i run it at 2% Agrose electrophoresis and it gave me a clear band as it appear in the lower line of the attached photo.
After digestion , the expected size is 260 .221. 40 bp but when I run it , I could not recognize the digested bands clear as in the upper line of the gel . does this problem relate to the digestion process or to the gel percentage ?
By the way I loaded 5 ul sample per well.