Rawan A El-Madhoun

3 Questions 15 Answers 0 Followers

Questions related from Rawan A El-Madhoun

How can I solve primer dimer & faint PCR product problem ?

My PCR product appear very faint with primer dimer in each samples , I tried to increase annealing temperature , the extension time and the initial denaturation temperature as recommended in PCR...

25 August 2015 5,098 16 View

How can I obtain clear digested PCR product bands in Agrose Gel electrophoresises?

I have a PCR product with 260bp size , usually i run it at 2% Agrose electrophoresis and it gave me a clear band as it appear in the lower line of the attached photo. After digestion , the...

30 December 2014 1,558 22 View

What are the causes of faint PCR product ?

I optimized my PCR Reaction and it gave me very good result , after that some PCR product samples start to appear very faint in comparison with the other samples and the product of the first...

22 December 2014 10,292 14 View