How can I measure Vitamin C Equivalent Antioxidant Capacity(VCEAC)per g of sample by reducing power assay?

More Sid Maelou Guzman's questions See All

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)?

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...

28 April 2024 1,522 0 View

Does anyone have a PDF file with the API Catalog (API systems from Biomerrieux) that are willing to share?

I have some environmental isolates that we are looking to identify. Morphologically, the majority of the isolates are Bacillus spp. We would like to use the API 50 CHB/E system and would like to...

28 April 2024 9,559 0 View

How can I make a baseline correction of a FT-IR?

Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...

25 March 2024 7,648 0 View

I'm struggling with RNA extraction from Avian Vaccins. Could someone Help me ?

I'm trying to extract RNA from Avian vaccines such Newcastle and Infectious Bronchitis, the brand of the vaccines is (VALVOC), so far I didn't extract any RNA,

27 November 2023 6,493 0 View

Why is my qPCR not working?

Hi y'all, I am running into some difficulties with qPCR (SYBR). To give y'all a brief background summary, I transfected some cells (ASO) and am running qPCR to see its effect on known genes using...

08 November 2023 9,775 6 View

Dear Researchgate community, i need an interpretation concerning my total DNA extraction ?

total Dna extraction from poultry tissue, kidney, liver, heart using spin column.

25 September 2023 493 6 View

What impact does the use of artificial intelligence have on the accuracy and speed of laboratory testing in clinical settings?

Medical laboratory technologists are likely to be faced with the challenges brought on by Artificial Intelligence (AI) as laboratory medicine continues to undergo digitization and automation. The...

02 September 2023 6,162 3 View

What do you think is the downside of artificial intelligence in the application to clinical laboratories?

Artificial intelligence may provide a lot of advance in the modern age, however, there is still some downside that AI has and lacks.

02 September 2023 1,126 2 View

Why grounded slot improve the gain and the efficiency of microstrip patch antenna ?

Hello I found in some publication that the gain and the efficiency of microstrip antenna could be ameliorated by making a slots in the ground. why the slots improve the gain and the efficiency...

15 January 2023 2,946 0 View

How to prepare a lactic buffer 1 mol/L for brucellose suspension ?

I want to prepare a Rose bengal test

16 December 2022 255 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does anyone know any C++ implementation of Kolmogorov-Arnold network other than mine?

Mine is sitting here: http://openkan.org/DLpiecewiseCPP.html I wish to see someone else's.

06 July 2024 9,974 1 View

Which is better for the student : Implementing the principles of object-oriented programming using Java or C++?

Object-Oriented Programming

29 June 2024 4,877 12 View

How could I determine antioxidant activity of Capsicum annuum fruits?

I need a detailed protocol of any suitable method for ex., DPPH to determine antioxidant activity.

28 June 2024 3,855 2 View

What is the exact mathematical formulae and the C++ code of EMMS drag model that is used and applied in ANSYS Fluent 2023?

EMMS - Energy Minimization Multi-Scale

18 June 2024 5,857 2 View

Can I dissolve DPPH in THF for antioxidant activity Assay ?

My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...

17 June 2024 4,183 3 View

Depletion experiments fo these three antioxidants in Atlantic Salmon?

Are there depletion experiments for these three antioxidants in Atlantic Salmon?

16 June 2024 1,915 1 View

Why does the antioxidant activity increase as the number of OH groups on the phenolic ring increases?

and Why do double bonds reduce it ?

13 June 2024 4,836 4 View

Please what should I do if I find a disorder when testing antioxidant activity by DPPH?

I have solubilized DPPH in methanol and used ascorbic acid as a positive control, and am testing the activity of pectins.

05 June 2024 6,197 1 View

How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...

05 June 2024 1,372 6 View

Bruce Neagle

The answer depends on your sample matrix. By I would suggest titrating a known amount of sample containing Vitamin C with Iodine solution as per USP. If this doesn't work you will have to develop a method by HPLC.

Mohammad Mehdi Ommati

Dear Sid Maelou Reambonanza Guzman,

Please read one of our manuscripts which we wrote about Total antioxidant capacity of liver entitled "The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of

Normothermic Recirculating Isolated Perfused Rat Liver "

Enclosed you will kindly find this manuscript.

I hope you can do it easily.

Regards,

Mehdi