How can I measure the ROS of bacteria ( E coli) using DCFH-DA? I am confused by reading different methods in papers and no one wrote the method very precisely. Is there any protocol for detecting ROS in bacteria since it's not adherent? Thank you.
I used ( Acrydine Orange / Ethidium bromide assay ) it simple and reliable methods by mixing the same volume from bacterial suspension with Acrydine Orange / Ethidium bromide. incubate at room temperature for 5 min. and then prepare slide smear from mixture leave to dry then examined using fluorescent microscope. survival bacteria were appear orange while dead bacteria take red color.