I need to determine the levels of metformin in plasm and the rate of excretion as well. Does it could be assessed by spectrophotometry? Or Are there other methods more reliable?
1. Nielsen, Flemming ; Christensen, Mette M. Brøsen, Kim. Quantitation of Metformin in Human Plasma and Urine by Hydrophilic Interaction Liquid Chromatography and Application to a Pharmacokinetic Study.Therapeutic Drug Monitoring: April 2014 - Volume 36 - Issue 2 - p 211–217
2. G.T. TUCKER, C. CASEY, P.J. PHILLIPS, H. CONNOR, J.D. WARD & H.F. WOODS. METFORMIN KINETICS IN HEALTHY SUBJECTS AND IN PATIENTS WITH DIABETES MELLITUS. Br. J. clin. Pharmac. (1981), 12, 235-246
3. Zhang X, Wang X, Vernikovskaya DI, et al. Quantitative determination of metformin, glyburide and its metabolites in plasma and urine of pregnant patients by LC-MS/MS. Biomedical chromatography : BMC. 2015;29(4):560-569. doi:10.1002/bmc.3314.
4. Bruce G. Charles, Noel W. Jacobsen,2 and Peter J. Ravenscroft’.Rapid Liquid-Chromatographic Determination of Metformin in Plasma and Urine. CLIN. CHEM. 27/3, 434-436(1981)
5. Eva Troja, Leonard Deda, Gëzim Boçari.Ion-pair HPLC method for the quantification of metformin in human urine. Vol.2, No. 1, Pages 16-24, doi: 10.17145/jab.16.004. (ISSN 2405-710X).
6. Patrick Nisse, Monique Mathieu‐Nolf, Marc Deveaux, Xavier Forceville & Alain Combes (2003) A Fatal Case of Metformin Poisoning, Journal of Toxicology: Clinical Toxicology, 41:7, 1035-1036, DOI: 10.1081/CLT-120026533
7. Chung H, Oh J, Yoon SH, Yu K-S, Cho J-Y, Chung J-Y (2018) A non-linear pharmacokinetic-pharmacodynamic relationship of metformin in healthy volunteers: An open-label, parallel group, randomized clinical study. PLoS ONE 13(1): e0191258.
8. L.BenziP. MarchettiP, CecchettiR.Navalesi. Determination of metformin and phenformin in human plasma and urine by reversed-phase high-performance liquid chromatography,