I have incubated the seed (25 deg C in dark) after imbibation. It was almost 144 h but no germination occurred. The main problem I think is the gelly surrounding the seeds.
Dear Mominul
Do the Hot water treatment 50 c at 20 to 30 min, then check the germination
Dear Dr. Chetan kumar M R: Thank you very much for your comments. OK, I'll try.
check priming time and optimize the temperature of germination.The optimal priming time varied with temperature.
Dear Dr Roberto Skwierinski: Thank you very much for your link. It will definitely helps me a lot, and are very interesting.........
please read work by the following authors embedded in the links:
1. http://www.jstor.org/stable/1935354; http://www.nrcresearchpress.com/doi/abs/10.1139/b85-116?journalCode=cjb1#.U71Y57Hm65o
2. http://pcp.oxfordjournals.org/content/12/4/567.abstract
3. Read also a book (available on google books) by Carol et al. (2014). Seeds: Ecology, Biogeography, and, Evolution of Dormancy and Germination
I hope you find this helpfull
See the article
SEEDS GERMINATION OF Hyptis suaveolens (L.) POIT IN FUNCTION THE
LIGHT AND OF THE TEMPERATURE.
http://periodicos.ufersa.edu.br/revistas/index.php/sistema/article/viewFile/556/437
ABSTRACT - This work aimed to evaluate the influence of light and temperature on germination of Hyptis suaveolens
seeds. The seeds were harvested at Mossoró/RN city and the experiment developed in at the Laboratory of Seed
Analysis of the Department of Agriculture of the Federal University of Lavras at Lavras/MG. The treatments was
consisted of four temperatures (20ºC, 25ºC, 30ºC and 20-30ºC) and two light conditions (light and darkness). The
treatments consisted of 200 seeds, with four repetitions of 50 seeds each, sown on a two Germitest towel paper,
displayed inside gerbox, and maintained for 30 days in germination chamber. A completely randomized constituted
design with 2x4 factorial design (two light expositions x four temperatures) was used with 4 replicates of 50 seeds. The
Scott-Knot’s Test at 5% of probability was used to compare the obtained averages. There was significant difference in
the interaction temperature x shines, in the two variables. It can be concluded that the largest germination of seeds
happened in the light presence and in high temperatures. And that the temperatures alternated of 20-30ºC, 30ºC and
25ºC are favorable for germination of seeds of Hyptis suaveolens.
Thank you very much Dr. Callistus Mpofu and Dr. Carlos Romero Martins for your kind suggestions. These informations no doubt help me a lot.
Kind regards,
A K M Mominul Islam
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