What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

02 December 2020 1,380 4 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

Which is the better procedure to revive pneumococcal strains stored at -80C?

after identifying pneumococcus bacteria, i store it using 30% glycerol at -80 C. One month later i tried to reisolate it. All i have is a sterile blood plate with no growth.

10 March 2020 1,650 2 View

How to conduct moderation analysis using PROCESS in SPSS with 2 categorical IVs, 1 continuous moderator and 1 continuous DV?

I'm using Hayes' PROCESS macro in SPSS to carry out a moderation analysis. I have two dummy variables (IV1 and IV2), one moderator variable (W) and one DV (Y). As the dialog box does not allow...

10 March 2020 6,652 3 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

Acidification of biochar to lower its pH?

I am trying to test my biochars (charred biomass residues) in ammonia absorption tests. The more acidic biochars (hydrochars) work better, they have a lower pH than the high temperature chars....

11 October 2018 181 7 View

Can you identify this insect ?

This insect was found in a rural zone (Ras Elma) in Moulares Gafsa Tunisia plz try to identify this insect at least ordre and family Regards

14 May 2018 4,298 8 View

What could be the cause of the double bands showing after running my PCR product on a gel?

After running my PCR on a gel, I got double bands showing for all the positive samples. However, the expected band was brighter than the other band I observed. The cycling conditions were 94 C- 5...

26 March 2018 1,676 3 View

What is wrong with my Western Blot detection?

06 June 2017 4,792 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View