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Questions related from Lydia Amari
I am using the ultracentrifugation method to isolate the extracellular vesicle, once I get the pellet I used to use 20 to 30uL RIPA solution to dissolve and collect the pellet. After protein...
18 January 2023 9,798 1 View
I am working on the isolation and caracterization of extracellular vesicles content by western blot technic. But I am not able to get for each experiment good concentrations for the EVs fractions....
18 December 2022 9,199 3 View
I've transfected fibroblasts with GFP tagging mitochondria and I collected the extracellular vesicles secreted . usually with my non transfcted cells, I used to see mitochondrial material in my...
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What concentration of camptothecin is effective to kill mouse fibroblast in 24h?
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Hello everyone, I would like to have recommendations to know which pertinent methods I can use to seperate extracellular mitochondria that are released in the media from cells and extracellular...
09 January 2022 6,230 2 View
Hello everybody, I am currently working on EV isolation and caracterization and exosomes by ultracentrifugation. I used beta actin as a loading control for my blots but i don't really know if it...
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I need to transfect MEF with GFP tagged for mitochondria complex III
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