How can I maintain the stability of polyacrylamide coated CE capillaries?

Martinas Urbonavicius @Martinas-Urbonavicius

13 October 2020 0 7K Report

I am using CE method to analyze proteins. The capillaries are coated with polyacrylamide. I am aware of that amide in PAA coating would have a pka value of 17 meaning that whatever buffer I use the amide functional group will always be more prone to gain a proton. Is there anything I can do to stabilize or regenerate the coating?

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