How can I know the mRNA quality, purified from cancer tissue?

More Samet Kocabay's questions See All

Can Anybody help me "which plasmids are suitable for gene cloning in lactobacillus species, AND How can I obtain it"?

In my PhD work, I want to clone some important genes in to the LAB bacteria but I dont know which plasmid use. ADDGENE good source for plasmids but I also need your help. Can Anybody help me...

31 December 2017 2,692 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Angelika Voronova

by measuring RNA integrity.

http://www.lifetechnologies.com/lv/en/home/references/ambion-tech-support/rna-isolation/tech-notes/is-your-rna-intact.html

Christophe B Poulet

The best would be to use the Experion (Biorad) or the Bioanalyser (Agilent).

Experion (Biorad): http://goo.gl/IOKEbI

Bioanalyser (Agilent): http://goo.gl/VlZQId

Then, an agarose gel is a traditional and a cheap method, but it uses more quantity of your sample.

Hope that's helpful.

Sibtain Afzal

Dear Samet

Plz read this chapter that i have downloaded for you from a book Ovarian Cancer.

best regards

i am attaching the book as well for your kind consideration.

Best Regards

Samet Kocabay

Thanks for helps...

Serena Di Cecilia

run a Bioanalyzer chip.