How can I know the mRNA quality, purified from cancer tissue?

More Samet Kocabay's questions See All

Can Anybody help me "which plasmids are suitable for gene cloning in lactobacillus species, AND How can I obtain it"?

In my PhD work, I want to clone some important genes in to the LAB bacteria but I dont know which plasmid use. ADDGENE good source for plasmids but I also need your help. Can Anybody help me...

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

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Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

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Universal bacterial primers targeting a mRNA?

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How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

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Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

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Angelika Voronova

by measuring RNA integrity.

http://www.lifetechnologies.com/lv/en/home/references/ambion-tech-support/rna-isolation/tech-notes/is-your-rna-intact.html

Christophe B Poulet

The best would be to use the Experion (Biorad) or the Bioanalyser (Agilent).

Experion (Biorad): http://goo.gl/IOKEbI

Bioanalyser (Agilent): http://goo.gl/VlZQId

Then, an agarose gel is a traditional and a cheap method, but it uses more quantity of your sample.

Hope that's helpful.

Sibtain Afzal

Dear Samet

Plz read this chapter that i have downloaded for you from a book Ovarian Cancer.

best regards

i am attaching the book as well for your kind consideration.

Best Regards

Samet Kocabay

Thanks for helps...

Serena Di Cecilia

run a Bioanalyzer chip.