How can I know the mRNA quality, purified from cancer tissue?

More Samet Kocabay's questions See All

How can i group my eeg electrodes?

Hey, I'm trying to group my electrodes in 2 or 3 groups. But I'm not sure that what is the best methods for this. This is my electrodes that ı recording: Fp1, Fp2, F3, F4, Fz, F7, F8, C3, C4, Cz,...

21 March 2023 5,781 3 View

About setting interstimulus interval duration as randomly in PsychoPy?

Hi, I'm trying to create an experimental paradigm by using PsychoPy. That is my question: I want to set the duration randomly between two stimuli (e.g., Blank500). More specifically, I will set...

22 August 2022 5,678 1 View

Convert data from NIC2 (Neuroelectrics,SP) into Brainvision Analyzer?

Hello everyone, I just recorded to EEG data by using Enobio32. And this data format is .edf and .nedf. How can i convert this file to .vhdr . Because ı want to import this data to Brainvision...

06 February 2022 6,511 4 View

Deep learning approach in neuroscience?

Hello everyone, Does anyone know of an application-based course or material that specifically examines brain images (EEG signals or fmri images) with artificial intelligence and deep learning...

26 July 2021 7,965 7 View

How to model new dampers in sap2000?

Hello friends, I read an article in which it introduced a new type of damper,The main idea of these systems is to combine two separate control devices with different strength and stiffness...

15 January 2021 2,832 2 View

I need Type-2 Fuzzy Logic documentation?

for FPGA implementation

06 February 2020 1,277 1 View

What could be the possible reasons for the difference between RT-qPCR and the comparative proteome analysis in procaryotes?

Suppose that after the comparative proteome analysis between two strains, I found one protein to be increased 5 fold in the mutant strain compared to wild type. Next I employ RT-qPCR to this gene...

27 July 2018 965 3 View

How to dissolve chitosan in sodium acetate buffer?

Hey there all, I need to prepare 0.5% chitosan substrate solution in 0.1 M Sodium acetate buffer (pH 5.6) for my enzymatic activity assay. The buffer is ready, the chitosan is ready (Sigma...

14 June 2018 3,164 3 View

Corticosteroid analysis from canine faecal samples - Lab recommendations please?

Hello I'm looking for a UK company that can analyse cortisol levels (corticosteroids) in canine faecal samples. Please can anyone supply recommendations? Many Thanks, Lauren

06 April 2018 3,560 3 View

How can I define non-linear spring behavior in abaqus?

I created a script file. But I can only define linear spring behavior. I want to define non-linear spring behaivor. mdb.models['Model-1'].parts['Part-1'].engineeringFeatures.TwoPointSpringDashpot(...

05 October 2016 9,168 6 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Angelika Voronova

by measuring RNA integrity.

http://www.lifetechnologies.com/lv/en/home/references/ambion-tech-support/rna-isolation/tech-notes/is-your-rna-intact.html

Christophe B Poulet

The best would be to use the Experion (Biorad) or the Bioanalyser (Agilent).

Experion (Biorad): http://goo.gl/IOKEbI

Bioanalyser (Agilent): http://goo.gl/VlZQId

Then, an agarose gel is a traditional and a cheap method, but it uses more quantity of your sample.

Hope that's helpful.

Sibtain Afzal

Dear Samet

Plz read this chapter that i have downloaded for you from a book Ovarian Cancer.

best regards

i am attaching the book as well for your kind consideration.

Best Regards

Samet Kocabay

Thanks for helps...