07 April 2018 3 4K Report

Hello I'm looking for a UK company that can analyse cortisol levels (corticosteroids) in canine faecal samples. Please can anyone supply recommendations? Many Thanks, Lauren

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Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

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How to perform a mixed-effects Poisson regression with mutliple DVs?

I have a set of 6 related likert-type DVs, a within-subjects time variable (i.e. each DV was measured at 3 time points), and a categorical between-subjects IV (condition). Is there a way to...

16 December 2020 9,798 10 View

Repeated measures ANOVA with technical replicates?

I am doing an experiment where I measure the blood pressure of a treatment and control group over the course of several weeks. At each measurement session I collect multiple recordings. From what...

06 December 2020 531 7 View

Top-coded age and regression?

What is the best way to deal with top-coded age while doing regression analysis. If you are examining the presence or absence of a disease as your outcome, and a number of independent variables...

13 October 2020 907 5 View

Is there a guide that will help interpret Ponceau S staining for western blots?

Greetings, I'm having difficulty understanding my results from doing a ponceau S stain on a nitrocellulose membrane after western blot. I loaded 10ug in lanes 2,4, and 6. 15ug in lanes 3,5,and 7....

22 July 2020 4,616 1 View

Developing Calprotectin Assay for Bovines- extraction buffer selection and centrifuge settings?

Hello, I am trying to adapt the human Calprotectin assay to use in bovines. For background, Calpotectin is a protein released by neutrophils following gastrointestinal inflammation. A couple...

16 March 2020 1,261 3 View

Are there additional practice datasets with code for Multiple-QTL Mapping in R/QTL other than the tutorial on the website?

Multiple-QTL Mapping in R/QTL

20 February 2020 1,469 1 View

Thickest sections possible for slide-mounted IHC of FFPE mouse brain tissue?

I am currently trying to section FFPE mouse brains using a rotary microtome for fluorescence IHC. I'd prefer to collect the sections free floating in buffer, stain them free floating and then...

04 February 2020 6,858 1 View

Antibody recommendation for IHC anti-IL18 staining in mouse brain free floating slices?

Hello, Many papers i look at include the santa cruz M-19 antibody which is now discontinued. I did find a few papers listing the R&D antibody raised against rat (AF521) but I believe this...

02 December 2019 8,578 3 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

18 August 2019 7,599 3 View

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