How can I isolate phospho serin276 NF-κB from nucleus of MCF-7 cells for analyzing with western blot?

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PPPPlease

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Divaker Choubey

The detection through immunoblotting is simple using phosph-specific antibody from commercial source using conditions recommended by the supplier. One would need to activate the NF-kB (p65) in cells using a stimulator.

Ashraf Alemi

hello Mr.Divaker Choubey

I sincerely thank you

Roberto Palacios-Ramirez

If you are interested in the nuclear expression you need to do a previous cell fractioning, you can find several protocols in the literature, I am leaving you one following the link

https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-3-294

Hello Mr.Palacios

Good luck