How can I isolate mouse pancreatic duct cell without antibody labeling?

25 May 2020 0 1K Report

I want to get some primary mouse pancreatic duct cells. However I can not use antobody labeling and FACS. How can I isolate pancreatic duct cell only through enzyme digestion?

Thank you very much!!!

Badges
Science topic

More My He's questions See All

An issue from another discipline：During the battery impact simulation, some units failed and penetrated. How can this be resolved?

LS-DYNA finite element simulation：During the battery impact simulation, some units experienced failure and subsequently underwent penetration. How can this issue be resolved?

24 June 2024 3,917 0 View

Why Q5 DNA Polymerase failed in PCR short primers?

Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...

17 June 2024 1,734 4 View

How to change my interest?

how do I change my interest in Researchgate? There are many notifications regarding articles, which I don't like at all. The website indicates they are according to my interest, but I can not...

11 June 2024 8,509 2 View

How can we use nanotechnology to better deliver drugs to cancer cells without harming healthy cells?

This question addresses the ongoing challenge of improving cancer therapies by focusing on the development of advanced drug delivery systems that can more accurately target cancer cells, thereby...

28 May 2024 6,197 5 View

I want this paper:Nonlinear hyperbolic equations and related topics in fluid dynamics?

who can send it to me?it is important for me.thanks.

14 May 2024 5,983 4 View

Why the precipitated RNA is in a liquid drop?

Hi, I have recently been working on RNA purification from RNA-binding proteins. I used a protein-specific antibody to immunoprecipitate the protein RNA complex and then extracted RNA from the...

01 April 2024 5,134 1 View

Code for PCA ?

Part of PCA code, I'm confusing about why need to decompose twice. for s=1:4 %Quadratic variation for each asset Var{s}=diag(diag(X{s}'*X{s})); %Quadradic correlation matrix...

01 April 2024 1,359 0 View

What is causing the NL-PSO in OriginLab to produce such large results (K2 and its SE) during fitting?

Hello everyone. I am recently working NL PSO fitting of my data in Originlab. NLPSO is the user-defined equation: qt = qe^2*k2*t/1+qt*k2*t I'm quite new to the adsorption study, therefore, I...

24 March 2024 2,925 2 View

Is there a clear version of "Theoretical analysis of relations between directivity of SAW transducer and its dispersion curves"?

I wonder if there is a clear version of "Theoretical analysis of relations between directivity of SAW transducer and its dispersion curves"? DOI: 10.1109/ULTSYM.1989.66961 In the current version...

19 March 2024 3,067 1 View

Why reducing the Al content in the coating can significantly increase its crystallinity?

I reduced the Al content in a high-entropy alloy coating and found that its crystallinity was significantly improved compared to before the reduction, and I would like to know why. In addition,...

10 March 2024 8,748 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

What additional processing or treatment options are available to enhance biogas plant digestate quality or value?

One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...

20 July 2024 3,392 2 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

From different feedstocks based Biogas digesters what would be the standard NPK - Nutrient contents of the digestate, and how consistent is it?

One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...

18 July 2024 9,907 12 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View