How can I isolate mitochondrial DNA from whole blood?

26 May 2015 1 6K Report

Anyone please help me by attaching the procedure on isolation of mitochondrial DNA from whole blood.

(i) extraction of an mtDNA-enriched fraction using a common plasmid miniprep kit and (ii) further purification of mtDNA using the Agencourt AMPure XP system. This method is capable of an up to ~2000-fold enrichment of mtDNA, is superior to commercial kits, and costs a fraction of the price.

BioTechniques, Vol. 55, No. 3, September 2013, pp. 133–136

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