Dear researchers,
how can I successfully isolate (primary oral squamous cell carcinoma) epithelial cells
I isolated epithelial cells very low because fibroblast cells overcame, although I used specific medium for epithelial culture.
Isolation, culture and analysis of mouse mammary epithelial cells.
Smalley MJ1.
Abstract
Limited understanding of the cell biology of the breast and breast cancer hampers our ability to develop new therapeutic approaches. Mouse models of mammary gland development and tumourigenesis are key to developing new insights into the biology of both the normal and diseased tissues. Recent advances have enabled the isolation, molecular characterisation and functional analysis of mouse mammary epithelial cell subpopulations from the normal gland, including subpopulations enriched for stem cell behaviour. Application of these techniques both to the normal mammary gland and to tumour models will promote a better understanding of the nature of the different epithelial cell types in the mammary gland, the origins of mammary tumours and the role of tumour stem cell
https://www.ncbi.nlm.nih.gov/pubmed/20204626
Hi Ali, thank you for posting tips and suggestions.
My question for you is:I separated epithelial cells by manual cell separation (MACS, CD326 (EpCAM)), but is very low and finally fibroblast cells overcome in dish culture.
I have actually tried this method, but eventually I had to loss the epithelial cells.
Can you explain in detail your protocol for epithelial separation from fibroblast and discard all of the fibroblast cells?
Thank you very much!
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