How can I isolate disease causing genes from fungus?

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What are the steps involved in conducting NMR measurements for polypropylene?

From literature I could see that I need a mixture of solvents to make solution of Polypropylene, But none of these solvents dissolve polypropylene at room temperature. How do I select a solvent...

15 January 2024 1,780 3 View

What is the role of antibiotics only (conservative) management of acute appendicitis in LMICs with poor access to healthcare?

Lots of studies in adults and children, mainly done in HICs, are advocating antibiotics only (conservative) management of acute non-perforated appendicitis. In LMICs, with poor access to...

10 November 2023 3,556 1 View

What exactly happens during alkali pretreatment (using NaOH or Ca(OH)2) and bleaching (with H2O2) of lignocellulosic biomass (agricultural residue)?

Does the alkali pretreatment followed by bleaching of biomass involve a certain chemical reaction or is it a physical process such as extraction? I want to know the exact mechanism of these...

03 August 2022 4,018 0 View

DOE based upon CCD RSM. All readings of 30 min combustion unsteady and non-normal(1800 reading points). Is averaging of data OK for further analysis?

Executing 5-factor 54 run Design of Experiment based upon Central Composite Design, Response Surface Methods and Desirability for robust parameter design . Each reading of 30 minutes combustion...

25 June 2018 2,369 5 View

Torsion Springs, if loaded in opposite direction to that of winding, deforms plastically and unable to give spring effect. How this can be formulated?

Torsion springs are always loaded in direction of winding so that it gives good spring back effect and desired force. If loaded in opposite direction, it tends to unwind the spring turns and...

05 March 2018 4,815 2 View

Limes flocculation and Limit of flocculation methods for the measurement of toxin concentration are same of different?

Limes flocculation test and Limit of flocculation test are used to measure the concentration of toxin or toxoid. What is Limes?

24 February 2018 1,690 1 View

How to regulate height, diameter and wall thickness in tropical sympodial bamboos?

what is the effect of 1. macro and micro nutrients, 2. sunlight and precipitation, 3. Canopy management and harvesting regimes, 4. Inter- and Intra-species resource competition (both within bamboo...

29 August 2017 4,780 2 View

How to calculate AUC value in Maxent?

I have generated a Maxent model for species distribution using only presence points. I need to evaluate the model and I don't know How to calculate the AUC values manually. I heard that the AUC...

07 May 2017 7,377 4 View

How to estimate copy number of ribosomal gene from whole genome sequence data??

I would like to estimate the copy number of the ribosomal gene from published whole genome sequence. Is there any direct method to do this??

12 March 2017 3,692 1 View

I am working on Brain tumor segmentation/detection using ANN,watershed and morphological operations but i lack images with grand truth any help?

I need small Training and test data with valid grand truth for my MS.c project regardless synthetic or real, if anyone can point me in right direction i would really appreciate it. "BRATS" isn't...

24 August 2016 2,913 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View