9 Questions 13 Answers 0 Followers
Questions related from Milind Mutnale
I have generated a Maxent model for species distribution using only presence points. I need to evaluate the model and I don't know How to calculate the AUC values manually. I heard that the AUC...
08 May 2017 7,319 4 View
some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...
27 October 2015 1,281 7 View
Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.
08 April 2015 8,155 10 View
I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method...
29 September 2014 7,858 3 View
I'm working on a disease causing fungus which infects only amphibians and I would like know which genes from fungus are responsible for disease... so to do this how can I proceed? any method or...
24 September 2014 6,134 3 View
I have very low amount of DNA 10 ng/ul. So if I would like to check on gel, how much ul I have to take?
10 September 2014 623 45 View
I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???
10 September 2014 3,617 4 View
I'm trying to extract Bd fungal DNA from a cotton swabs after swabbing on frogs. I'm trying to get total DNA, but I'm not getting it. Right now I'm using lysis buffer containing EDTA, Tris HCl,...
08 September 2014 3,999 6 View
I'm face one problem, that is while taking readings on nano-drop for DNA concentration, I'm getting negative reading. I'm taking same solution in which I have dissolved my DNA so can anybody tell...
08 September 2014 1,581 5 View
