Milind Mutnale

10 Questions 13 Answers 0 Followers

Questions related from Milind Mutnale

How to calculate AUC value in Maxent?

I have generated a Maxent model for species distribution using only presence points. I need to evaluate the model and I don't know How to calculate the AUC values manually. I heard that the AUC...

08 May 2017 7,339 4 View

How to estimate copy number of ribosomal gene from whole genome sequence data??

I would like to estimate the copy number of the ribosomal gene from published whole genome sequence. Is there any direct method to do this??

13 March 2017 3,671 1 View

Why I get weird curves in qPCR?

some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...

27 October 2015 1,298 7 View

Can we use regular PCR master mix for qPCR assay?

Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.

08 April 2015 8,163 10 View

How can I find out disease causing genes in fungi?

I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method...

29 September 2014 7,878 3 View

How can I isolate disease causing genes from fungus?

I'm working on a disease causing fungus which infects only amphibians and I would like know which genes from fungus are responsible for disease... so to do this how can I proceed? any method or...

24 September 2014 6,176 3 View

What is the minimum DNA quantity (ng) required to visualize DNA on Agarose gel?

I have very low amount of DNA 10 ng/ul. So if I would like to check on gel, how much ul I have to take?

10 September 2014 638 45 View

How many pUC18 plasmid DNA molecules will be there in 10 ng/ul conc.?

I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???

10 September 2014 3,633 4 View

Can anybody tell me which is the best DNA extraction method for total DNA extraction from cotton swabs?

I'm trying to extract Bd fungal DNA from a cotton swabs after swabbing on frogs. I'm trying to get total DNA, but I'm not getting it. Right now I'm using lysis buffer containing EDTA, Tris HCl,...

08 September 2014 4,013 6 View

Does anyone have information negative nano-drop reading?

I'm face one problem, that is while taking readings on nano-drop for DNA concentration, I'm getting negative reading. I'm taking same solution in which I have dissolved my DNA so can anybody tell...

08 September 2014 1,585 5 View