How can I isolate a virus from lung tissue of pigs?

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Labeled datasets for age classification?

Dear researchers, I am working on age classification of audio content, I need some labeled public datasets to train my model. Could anyone help? Thank you!

17 May 2021 7,558 4 View

How separate calcium chloride CaCl2–water mixtures?

Hi all, I am going to separate calcium chloride CaCl2–water mixtures. Given that, the concentration of CaCl2 is 7%. There any suitable method for separation and extraction of CaCl2?

26 April 2021 2,918 10 View

Why I couldn't obtain a gel from APTES? Does the Amine group affect the gelation process?

I am trying to make a gel from APTES as a precursor using the sol-gel reaction. I tried different solvents (ethanol, acetone, DMF) and different catalysts (basic and acidic) with different...

03 October 2020 9,934 5 View

How to estimate Lipid-Protein interactions durations from MD trajectories ?

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26 October 2019 5,506 3 View

I have a DCP di calcium phosphate and phosphoric acid, and I want to synthesis the triple super phosphate (TSP). How can I do please?

I have a DCP di calcium phosphate and phosphoric acid, and I want to synthesis the triple super phosphate (TSP), from this two reagents. How can i do please. Thinks a lot for your help

13 October 2019 7,130 1 View

Why we use the Bose-Einstein population in Raman spectrum?

In the temperature dependence of Raman scattering, why we use the Bose-Einstein population in Raman spectrum? and what is the purpose of this term?

26 June 2019 4,180 2 View

How can we separate between the fluorescent effect and second-order vibration in the wavenumber [1000-2000cm-1] in Raman Spectroscopy?

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30 April 2019 9,462 8 View

How to read Gaussian Cube file?

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21 April 2019 8,568 1 View

What is the difference between 633nm and 785nm lamp in Raman Spectroscopy?

I am researching rare earth doped pervoskite ceramics and found that most researchers used 633nm Laser. What is the difference between them and will there be much difference in the results?

15 April 2019 5,646 8 View

Can anybody give me a protocol to measure NK cell activity?

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30 January 2019 1,749 5 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

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04 August 2024 9,913 7 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

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03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

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01 August 2024 589 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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01 August 2024 2,079 0 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Wenfang Xie

The susceptibility of MDCK cell to influenza virus decreases with the generations.So why not choose cell lines from the early passages.Good luck!

Thomas Rowe

How are you trying to isolate your virus? Make sure that you add TPCK-Trypsin to your media at @2ug/ml. Please describe more of how you are trying to isolate your virus (cell line, medium, methodology, etc.)

Pierre Lebon

, even if you have no CPE test the cells with monoclnal Antibody by immunofluorescence

You can try isolation with HuH7 cells