Ideally peaks should be separated and having a somewhat symmetric pattern but could any one give me a help with reading H NMR with multiple peaks totally mixed up which could not be attributed any particular group?
Dear Sir
hopefully, u can try these approaches ....
1. use a solvent without(non-water base) water or don't use water in ur metabolite
2. use a D2O NMR spectrum of ur sample to examine the quantity of water that is present in ur sample.
3. use a temperature dynamic spectroscopy to higher boiling temperature of the water if ur metabolite is stable in this temperature. in this case as the same time the spectra are record, water present in ur sample evaporates and u can remove the overlapping. you can go through these link and article ....although the paper is based on HOHAHA
https://spin.niddk.nih.gov/NMRPipe/doc2new/
Dear Abdollah, can you show the spectrum...at least I can not see any data in your post.
Kind regards
Alfred
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