How can i induce experimental salmonellosis in mice?

More Jinan Abdul-Amir Sabeeh Al-Hussaini's questions See All

What are the thermophysical properties of a DEMINERALIZED WATER?

Please someone help me with them.

24 January 2024 761 6 View

Unit conversion for Isobaric specific heat (Cp)?

Hello everyone, please how can I convert J/mol/K to J/kg/K? Thank you in anticipation of your good response.

17 December 2023 2,732 4 View

How to calculate Wall heat flux (W/m-2) when you have heat input (W)?

A constant heat flux 172.87W was given to 2-D wall of 200mm high with outer diameter of 22mm and inner diameter of 20.2mm (that is 0.9m is the wall thickness). Please how can I convert this...

21 October 2023 5,564 5 View

Can we study drought tolerance, yield and some diseases observations in the same field trial (RCBD factorial) on wheat genotypes?

There are 10 Varieties of wheat for drought selection, beside this we want to know the most productive verities + diseases infections in open field trial in the same experimental design. Thanks...

03 April 2023 1,663 3 View

Dummy variable in long-run,ARDL?

I have run a dummy variable in my ARDL but it hasn't stimated in long-run after that it cames in short one. Would you mind helping me to add my dummy in long-run too?

21 July 2022 4,968 0 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 10,151 2 View

Dear Colleagues, how can I convert biomass from gram(g) to tkm-2 ?

The data is from a different source and in different units, I want to convert the biomass from gram (g) to tkm-2 before transferring the data into the Ecopath with Ecosim software.

03 October 2020 7,125 3 View

Can the Universe be finite and unbounded and still be Euclidean?

According the current Cosmology our Universe is finite and unbounded, even if it is expanding. The visual analogy that is given to explain this is an expanding sphere where the galaxies are dots...

08 September 2018 9,896 39 View

Why does not codeine produce analgesic effect when taken by a patient suffering from dental pain?

codeine is an opioid drug

12 October 2017 8,446 2 View

Why does not codeine produce analgesic effect when taken by a patient suffering from dental pain?

codeine is an opioid drug

09 October 2017 9,530 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Jabbar A A Al-Sa'aidi

Dear

You can read Zina Hamza MSc thesis under my and Dr. Mohsen Alrodhan supervision.

Best Wishes

Thanks Dr. Haiffa

Kind regards

Ibrahim Alrashid

Dear Dr , I believe Dr Mohammed Alameri he was interst in induce Salomenella infection

Basima Jasim Mohammed

Hi DR. This link can help you.https//www.ncbi .nim.nih.gov.