How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

Why does not codeine produce analgesic effect when taken by a patient suffering from dental pain?

codeine is an opioid drug

12 October 2017 8,330 2 View

Why does not codeine produce analgesic effect when taken by a patient suffering from dental pain?

codeine is an opioid drug

09 October 2017 9,406 2 View

What is effective dose of garlic powder when used as feed additive for poultry food?

garlic powder

03 April 2017 2,574 2 View

What is the scientific method for preparation of penicillin in the lab of undergraduate students?

methods with references please

03 April 2017 5,578 1 View

What are the best methods used for identification of glycosides in herbal extracts?

i found this one: 2 ml plant extract was added to 2 ml ofchloroform. Carefully added 2 ml of sulphuric acid and shakengently. A reddish brown colouration was indicated the presenceof a steroidal...

19 March 2017 1,290 5 View

What are the recently used herbal medicines for management of H. pylori infection?

02 March 2017 5,984 3 View

KIRBY-BAUER chart for sensitivity and resistance of bacteria to nearly all the known antibiotics?

02 March 2017 7,574 6 View

What properties in drugs make them addicting?

those drugs act on the brain in different ways

02 March 2017 510 0 View

How can i prepare antibiotic discs from medicinal plant extract?

these discs are used for evaluation of sensitivity test in vitro

02 March 2017 1,565 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View