I recommend you to explore the use of PEG as osmoticum and potting the plants in dialysis membranes. in this way, by choosing the adequate concentration and molecular weigh of PEG you can induce the osmotic potential of the subtratum. Everytime you apply water to your potted plants, osmoticum will substract the amount of water need to reach the equilibrium in the substratum.
Many other people (including us) have used another methods, more simple than this but not as accurate. For instance, by keeping constant the weigh of the potted plant once recahed the desire RWC or water potential.
I think, to induce drought stress in Sorghum under potting soil, you have to grow sorghum plants under high temperature, high intensity of light and receiving dry wind. These are the three major factors which causes drought stress.
I recommend you to explore the use of PEG as osmoticum and potting the plants in dialysis membranes. in this way, by choosing the adequate concentration and molecular weigh of PEG you can induce the osmotic potential of the subtratum. Everytime you apply water to your potted plants, osmoticum will substract the amount of water need to reach the equilibrium in the substratum.
Many other people (including us) have used another methods, more simple than this but not as accurate. For instance, by keeping constant the weigh of the potted plant once recahed the desire RWC or water potential.
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