One of the the weakness of mini column methods for DNA extraction is the very low stability of extracted DNA using guanidium salts which is sometimes maximally 1 week in -20c . How can increase the stability of DNA for at least 6 months or 1 year ?
We never encountered any degradation problem that would be specific to minicolumn-purified DNA. It sounds like you have got some nuclease contamination in your buffer(s). I would make them fresh and autoclave them or start with a new kit. Also, work with autoclaved plasticware (pipette tips).
We never encountered any degradation problem that would be specific to minicolumn-purified DNA. It sounds like you have got some nuclease contamination in your buffer(s). I would make them fresh and autoclave them or start with a new kit. Also, work with autoclaved plasticware (pipette tips).
Dear Gholamreza Taheripak
I want to know that are you using kit or manual method for DNA extraction and what buffer are you using to reconstitute the DNA and pH of this buffer. The suggestion given by @Pierre Béguin is sound good make fresh buffer with slightly basic pH.
Dear Pierre Béguin
Thanks for your answer. I will check the items you mentioned. Is it possible it would be as a result of using old columns ?
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