Ks Lim

4 Questions 11 Answers 0 Followers

Questions related from Ks Lim

How can I improve RNA purity?

I have use Trizol method to extract my RNA from cancer cells. The A260/280 ratio range is within 1.8-1.9. Would that be possible to increase the value from 1.8 to more than 2 by re-precipitating...

05 May 2015 4,601 6 View

Can anyone help with cell morphology and passage number?

I am currently doing EMT study. I notice that cells tend to grow in spindle shape after few passages, which are resemble to the EMT-ed cells. What are the possible factors that contribute to this...

07 July 2014 846 1 View

Can formalin mix with Triton-X in PBS to make 3.7% formalin with 0.1% Triton-X solution for intracellular staining?

A standard protocol for intracellular staining usually separate the fixation and permeabilization part. Can I do the fixation and permeabilization at one time by incubating cells with 3.7%...

07 July 2014 6,236 7 View

Can anyone help me with these unusual results when multicolor staining in flow cytometry?

Currently, I am doing a research on NKG2D ligand expression on cancer cells. I would like to use dual color staining, which are APC-conjugated Ab for ULBP-1 and PE-conjugated Ab for ulbp-2....

03 March 2014 8,000 20 View