I usually extract RNAs from tissues (muscles) by TRIzol, which I consider a very efficient extraction method. However, the obtained RNA is often inpure (low 260/230 levels) and amplification in negative controls often occurs in my qPCR experiments, too.

I tried to proceed TRIzol-extracted RNAs to columns-based kits (e.g. Macherey-Nagel), but this procedure extremely reduced RNA yield. So how can I improve RNA purity without reducing its yield? Even when 260/280-260/230 ratios are good, amplifications in negative controls occur...

Thanks in advance for your suggestions.

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