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Questions related from Chiara Benedetto
Dear all, I am still facing a lot of troubles with my RNA extraction by TRIzol. Despite my attention in avoiding any kind of contaminations (especially with the interphase/organic phase),...
01 January 2018 4,072 5 View
Do you have any experience with RNA clean-up by Direct-zol kit from Zymo research? Since my TRizol-obtained RNA was full of contaminants, I tried to clean it up by first resuspending it in an...
01 January 2018 9,127 7 View
01 January 2018 6,934 2 View
Hi all, I have tried to clone a 500 bp sh-insert into a pSMD2 vector (4830 bp) for so long, but every time the mini-preps check is not successful. Digestions give the right vector band but never...
10 October 2017 2,113 4 View
I usually extract RNAs from tissues (muscles) by TRIzol, which I consider a very efficient extraction method. However, the obtained RNA is often inpure (low 260/230 levels) and amplification in...
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