How can I improve on the quality of DNA isolated from the tobacco aphid Myzus p. nicotinae?

More Rhoda Masukwedza's questions See All

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 7,213 5 View

Causes of poor cell viability of WBCs after RBC lysis with 1X FACs Lyse buffer?

We are performing a whole blood lysis procedure on heparinized blood (3mL) to lyse RBCs and later remain with WBCs that we count and freeze down and later cryopreserved in liquid nitrogen for...

20 October 2020 7,900 6 View

What are the most relevant parameters to measure as soil quality indicators?

Parameters that one can use when doing a conservation agriculture study

30 August 2020 2,423 16 View

Given the complexity of bio-cultural diversity, what images come to mind in our landscapes and lives?

Did you know there are over 6900 languages in the world with a strong correlation between language loss and biodiversity loss? And the exciting thing to me is that if my language frames my...

19 November 2015 6,711 14 View

Does aflatoxin grow during grain storage? At what rate and what catalyses the process?

31 July 2014 7,374 6 View

Can someone share some information on food fortification?

I am doing a lot of research about food fortification: Corn, Sorghum and Soybeans plus other things to make a porridge flour for children, lactating mothers and adults.

07 May 2014 6,134 5 View

Vehicular emission and cancer?

Is there a proven relationship between vehicle emission and cancer diseases?

04 February 2014 7,775 13 View

What opinion do you have on modern incineration? Is it a technology that should be embraced world wide to tackle the waste management crisis?

Waste-to-energy or recycling or composting?

10 June 2013 9,000 8 View

Can cells with CCR5 delta 32 (mutated form CCR5) receptors be isolated from immunized individuals , cultured and given out- to help fight HIV virus?

A step in developing vaccine for HIV virus.

01 January 1970 5,472 3 View

How do you feel about faith healing?

Any opinion, any view or any experience. No need to worry about etiquette or spirituality, just exclaim your thoughts with righteousness in mind. And the kids.

01 January 1970 9,941 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Nadine A. Gund

Hi Rhoda, I worked with aphids in cereals. I used this kit and had high amounts of extracted DNA: MasterPure from Epicentre. Cheers, Nadine

http://www.biozym.com/DesktopModules/WebShop/shopdisplayproducts.aspx?id=154&cat=MasterPure+DNA+Purification+Kit

Manoj Rajput

use QIAGEN PLANT genomic DNA extraction kit

Vincent Lefebvre

It is for aphids and not for plants, so I would rather recommend the Qiagen Blood & Tissue DNA extraction Kit. I use it for many insects and the results are great most of the time. Good luck !

Sascha M. Kirchner

Hi Rhoda, I used the Qiagen Blood & Tissue DNA extraction Kit, too - with moderate success. You could also try the attached protocol that should work for aphids.