Can cells with CCR5 delta 32 (mutated form CCR5) receptors be isolated from immunized individuals , cultured and given out- to help fight HIV virus?

More Rhoda Kwakyewaa Antwi's questions See All

Integrating Bayesian Network Model with GIS?

I have modeled coastal vulnerability in GeNIe and I want to know how to integrate Bayesian Networks with GIS (Geographic Information Systems). Or are there r or Python or any other language...

17 September 2023 9,244 1 View

What is a cottage, village, town, hamlet and a city?

their meaning populations etc

05 March 2023 2,296 3 View

Is it possible to design and build habitable structures on water that are powered by hydroelectric turbines beneath them??

Building habitable structures on water is an interesting concept that has been explored in various ways, from floating homes to entire cities built on water. While there are many engineering and...

17 February 2023 6,561 1 View

Moderation or mediation analysis?

The researchers hypothesise that the more green space a mother’s local area the fewer depressive symptoms they will report. However, the effects of living near to green space would depend to some...

11 January 2023 1,525 5 View

Can I get an in-depth explanation of capabilities-based theory( dynamic and operational capabilities)?

My research question is identifying the capabilities of food supply chain actors and how these capabilities affect the reduction of food loss and waste.

30 August 2022 6,965 2 View

Input Files Examples or Tutorials in PhreeqC??

Dear all, I'm having challenges with modelling the reactions between H2 gas, brine and reservoir minerals in PhreeqC. I'd appreciate examples of Input files or Tutorials. Thanks.

24 August 2022 1,518 0 View

Flac 3D 6.0 for dynamic analysis?

I'm Performing slope stability analysis using FLac3D. 6.0. the constitutive and seismic waves seem correct, but the software still refuses to perform

08 July 2021 5,837 6 View

How can silica content in rice husk ash (RHA) be quantified analytically without instruments?

i want to know how to determine the percentage of silica in rice husk ash produced at optimum conditions without the use of XRD and XRF but perhaps through titration and other analytical methods

06 April 2021 4,039 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 7,213 5 View

Causes of poor cell viability of WBCs after RBC lysis with 1X FACs Lyse buffer?

We are performing a whole blood lysis procedure on heparinized blood (3mL) to lyse RBCs and later remain with WBCs that we count and freeze down and later cryopreserved in liquid nitrogen for...

20 October 2020 7,900 6 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Does the delta function actually make sense to use on point particles?

The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.

31 July 2024 10,109 3 View

How to find a reaction is reversible or irreversible in CV? How to know the number of electrons transfer?

My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...

30 July 2024 4,671 3 View

Please investigate the effect of temperature and strain rate of hot deformation and the formation of delta ferrite in martensitic stainless steel 410?

25 July 2024 9,408 0 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

How does that stream's gradient change downstream as it enter the alluvial fan and difference between an alluvial plain and a delta plain?

12 July 2024 7,337 0 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

د.باقر فليح عبد الحسن

آسف السؤال ليس من اختصاصي

Narayanan Kalyanaraman

Dear Rhoda,

It is a good view. However there are dozens of technical limitation to begin such therapeutic or preventive procedures at large scale. Technically, some of the procedures is in practice. Please follow the links

Article Long-term control of HIV by CCR5 Delta32/Delta32 stem-cell t...

http://www.bloodjournal.org/content/117/10/2791?sso-checked=true

Article Stem cell transplantation in strategies for curing HIV/AIDS

Florian Mutasingwa

It is a new observation that needs to be reviewed and considered for trials