Can anyone please help me out on gDNA isolation from a single aphid, which method would best suit for extraction from a single aphid? Currently, I used salt extraction method (required 2 days) and TaKaRa MiniBest Universal Genomic DNA Kit, the results were vague and time taking.
I agree with Harald, Qiagen has kits for DNA extraction and they work fine. Check this paper: Article A new non-destructive DNA extraction and specimen clearing t...
Thank you Respected Kuhnel and Gosse for your answers. I lysed single aphid in liquid nitrogen thoroughly and started pipette in lysed buffer. Well, I must try Qiagen kit.
Hi Umair
Here we also use Qiagen and TIANamp kit. I used both and I think they have equal output. According to the protocol, 3 hours is enough for tissue lysis, but I recommend longer time and even over night.
Good luck
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