Muhammad Umair Sial

3 Questions 3 Answers 0 Followers

Questions related from Muhammad Umair Sial

Why is my LAMP reaction not turning violet to sky-blue after adding HNB?

I am working on SNP through LAMP, but facing few issues on false naked eye detection in positive samples. I have checked and detected SNP through PCR by running F3 and B3 primers and even got...

12 December 2018 8,783 3 View

Hi ! Could anyone help me out in designing LAMP primers? I tried many times but my JAVA applications is stopping to display the results.?

Attached is the sequence file of my gene and my target region is in first 400bp. Many thanks for consideration.

01 January 2018 2,504 4 View

How can I improve gDNA quality extracted from a single aphid Myzus persicae?

Can anyone please help me out on gDNA isolation from a single aphid, which method would best suit for extraction from a single aphid? Currently, I used salt extraction method (required 2 days) and...

01 January 2018 2,920 3 View