Dear all,
I am studying embryo developmental stage of flowering plant with paraffin method. However, I can't still identify the prior stage of globular. How can I search the early stage, especially zygotic stage.
Dear Scientists and Colleagues, I trust this message finds you well. I am reaching out to seek your valuable expertise and guidance on a matter related to my current research. My focus revolves...
16 February 2024 8,137 2 View
Hello, I conducted mouse tumor digestion using two different protocols: one involving 0.1% trypsinization and the other using 0.4 mg/ml collagenase + 1.25 U/ml dispase II. The chopped tumor was...
22 November 2023 8,055 0 View
I would like to observe myosin heavy chain and myosin expressions in C2C12 3D samples. What is the least amount of cells required for Western blot? If I start with 10000 cells, will the results be...
23 October 2023 4,746 0 View
Here i need assistant of coming up with the best and easiest project for my final year
25 September 2023 4,571 0 View
Leading skills include the ability to inspire and motivate teams, fostering a collaborative and high-performing work environment. Employees with leading skills can effectively communicate...
30 June 2023 4,696 3 View
For analysis of C2C12 myoblast cell differentiation in Cell Profiler Software.To assess Myosin Heavy Chain expression in differentiated C2C12 cells.
10 April 2023 4,523 2 View
Dear everyone, I have obtained the lead-isotope compositions of galena, but I am not sure how to determine its model age. Could someone please assist me by providing step-by-step instructions on...
08 March 2023 8,030 0 View
Hi all, I am doing a series of large-scale experiments to extract HLA class I peptides. After the desalting step, I elute the peptides with 50% ACN instead of 30% like most people do. The idea is...
25 February 2023 977 0 View
Dear all, I am making the all-dielectric metamaterial structure, and got trouble with the input thickness for dielectric substrate (e.g. sapphire). When I change the thickness, the noise (Fabry...
18 October 2022 5,924 0 View
Hi folks, Currently running some convergence tests for magneto-crystalline anisotropy energy (MAE) of Fe (e.g. E_111 vs E_110 vs E_001). As you know calculating MAE requires very strict...
17 May 2021 1,358 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...
28 July 2024 8,169 0 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??
19 July 2024 8,571 1 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...
30 June 2024 2,891 1 View
I am looking for a methodology to evaluate the quality of wastewater using zebrafish larvae and embryos. Test No. 203: Fish, Acute Toxicity Test generally describes the procedure, but I have...
16 June 2024 6,473 0 View
I want to teach a class using zebrafish embryos. It's in the afternoon, so I want to get my hands on some fake, reusable embryos that students can use for practice. Does anyone know of any?
10 June 2024 3,956 1 View