Please I found it very difficult harvesting H37Ra cell I cultured on LJ slant.Each time I tried to scrape out the cells with a loop ,the green coloured medium followed so much that I could not harvest pure cells.

So I decided to homogenise /sonicate every thing hoping that during lysis only bacteria cells will be lysed and I could get the material of my interest in the supernatant.Is this approach right?Can this affect my result?