Cyril Alozieuwa

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Questions related from Cyril Alozieuwa

How can I harvest pure cells from LJ culture slant without scooping the medium with the cell?

Please I found it very difficult harvesting H37Ra cell I cultured on LJ slant.Each time I tried to scrape out the cells with a loop ,the green coloured medium followed so much that I could not...

10 October 2018 4,950 0 View

Why was there no separation of phases as I expected with hot phenol?

I am carrying out delipidation on an extract of H37Ra.I added hot phenol, after 1 ,I centrifuged at low speed but there was no separation of aqueous phase from the phenolic phase.I had thought...

08 August 2018 3,748 0 View