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Did anybody have an issue of not picking up variant in Nested fragment in one PCR conditions and picking it up in the other? By PCR conditions I mean different Tag Polymerase used. The variant...
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I am using LR-PCR fragments as a template for Nested PCR. After performing Nested PCR and sequencing on one of the LR-PCR products I observe low intensity substitutions and deletions which do not...
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