How can I get rid of abundant protein (albumin, immunoglobulins) from exosomes isolated from platelets after ultracentrifugation? I need a simple, quick, and cheap solution.
This process is used for both our custom antibody expression service, as well as to produce all the reagents in our rapidly growing catalog of recombinant antibodies and proteins.
Hi Joanna, any post-isolation treatment, such as washing, risks significant loss of your exosomes. There are no "simple, quick and cheap" methods that are effective.
You could try diluting your sample with buffer before ultracentrifugation to reduce the concentration of the unwanted proteins.
Hi Joanna,
see if the amicon ultra centrifugal filters can be used in your case. I used to use it to concentrate HIV-1 particles, and they kinda have the same size as exosomes.
There are gels to remove albumine from serum samples for proteonics studies, check whether these can be used for exosome preparations. Alternatively, spin the exosomes through a sucrose cushion, soluble proteins should stay in the sample zone. With metrizamide such cushions can be even isotonic, but it is much more expensive.
IgG and albumin depletion kits are provided by Sigma. They are quick and effective and regularly used for proteomics work. I have used them in studying low-abundant proteins. But need to check if it works for exosome related your project.
Creative Biostructure can provide a variety of exosome isolation methods, which can achieve successfully separation of exosomes from proteins and other macromolecules. I've worked with this CRO before, maybe you can try it.
https://www.creative-biostructure.com/exosome-isolation-and-purification-650.htm
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