04 January 2018 4 2K Report

How can I get good PCR protocol for the exon4 of LDLR gene?

More Shima Parviz's questions See All
For Amyloid beta ELISA should i use the supernatant or the cells?

i am planning to do ELISA for AB quantification in an Alzheimer related project. the sample is cell culture (not animal samples), since AB deposits in the extracellular environment should i use...

14 May 2024 5,244 3 View

How can I train the proposed model using a high-dimensional tensor?

Hello, dear researchers I hope you are doing well I have a high-dimensional tensor: 700*700*700 How can I train the proposed model with these dimensions? The predictive model is also a...

09 March 2024 6,557 0 View

How do I design MTT for enaluation of growth factor effcts for fast growing cells?

Hello everyone I am trying to design an MTT experiment for an immortal adherent cell line with doubling time of 20 hours. The goal is to evaluate effects of certain growth factors and their...

13 February 2024 3,162 4 View

What is the preferred medium for expansion of Mesenchymal Stem Cells?

I have read some papers on MSC isolation from human adipose tissue. DMEM and DMEM/F12 medium have been used for expansion. I need to know which type of DMEM (low/high glucose) is preferred for MSC...

05 February 2024 9,290 6 View

Is it a bioinformatics tool for extracting 3D coordinates?

Hello, dear researchers I hope you are well Is there a tool that gives 3D coordinates for each amino acid? The protein sequence is in FASTA format An example sequence...

31 January 2024 1,375 7 View

Is GAN Network as sample generator?

Hello, dear researchers I hope you are well Please introduce a reference that shows the role of the GAN network as a sample producer or sample generator It means that the GAN network balances a...

29 January 2024 9,977 0 View

Precision , reconition rate, and accuracy ?

HELLO DEAR reseacher what is different between Precision , reconition rate, and accuracy ? Plese introduce your explaination based on proper references ? Thanks in advance

18 January 2024 2,135 3 View

Pixel coordinates using distance coordinates in MATLAB or python?

DEAR Reaseher I have question in machine learning How to get pixel coordinates using distance coordinates in MATLAB or python?This means that we have distance coordinates such as...

15 October 2023 8,904 7 View

Which technique is suitable for imbalanced dataset?

Hello dear researchers There is a data set with a blessing rate of 1 to 17 means that the number of negative classes is 17 times the number of positive classes To address this problem, sampling...

18 September 2023 5,036 3 View

What is the proper solvent for super absorbent polymer (sap)?

I need to dissolve sap, what is the proper solvent?

20 August 2023 7,831 1 View

Similar questions and discussions
How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

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