19 August 2021 0 4K Report

I use 1-2g fresh surgical samples of endometrial cancer patients and temporarily save it in MACS® Tissue Storage Solution(Miltenyi company), then I digest the tissue in 10%FBS 1640 medium with 1 mg/ml collagenase Ⅳ and 0.5 mg/ml collagenaseⅠ for 1 hour. After that, I filter the suspension with 40 μm strainer, undergo RBC lysis and use 2 centrifuge tubes of 3ml 40%/3ml 70% percoll to acssess lymphocytes lyer. However, I only get 1-2 * 107 cells, and when I continue to use CD8+ positive selection beads to get CD8+T cells via a magnetic column, I can not collect enough T cells for next funtional experiments.( I need at least 3*106 CD8+T cells for functional usage).How can I Optimized experimental scheme to get more CD8+ TILs?

More Xinyue Ma's questions See All
Similar questions and discussions