How can I get crude extract from onion and what kind of Muscranic agonist can be in onion?

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Nallely Luviano

I have a method to extract and quantify allicin

Kehinde Alare

Oh, are you willing to share?

Carlos Alberto Lobato-Tapia

You can use a bio-directed study. It can be by column chromatography or with a preparative HPLC. The fractions that you are obtaining are tested with your biological model that evaluates the cholinergic agonist effect. The fraction that is most active is again semi-purified by these two techniques (CC or HPLC), so until you obtain and separate your active compound.

Regards

Thanks