I am using a reference "Jia-Long Yao, et al. (2013) Plant Cell Rep. 32: 703-714".
I am now observing whether it is rooting or not. Please tell me the good advise what to do.
Hi! Check the following links you get useful information.
What hormones you have to add for shoot multiplication and root developing.
A New Method for Rapid In Vitro Propagation of Apple and Pear
http://hortsci.ashspublications.org/content/36/6/1102.full.pdf
In vitro cloning of apple (Malus dometsica Borkh) employing shoot tip cultures of M9 root stock
http://nopr.niscair.res.in/bitstream/123456789/7769/1/IJBT%205(4)%20543-550.pdf
Google it just by typing apple tissue culture you get vast information.
rooting in IAA or IBA (0.2-1.0 mg/l) in half strength MS medium may be helpful..
Thanks a lot. I am trying these methods. I hope it is very advanced and it is going well from now. T. Momma, Ph.D.
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