You can used foods source for isolation examples (milk , milk production, fruits and water) ,It was temperature treatment (80 c at 30 min) and cultured in nutrient agar in 32 c at 24 hours .
Thank you Ido Hatam sir, but their (ATCC) cost for the bacterial stain is so high that I can't afford.Please suggest me any name from where I can get it as gift or at very reasonable rate .
You should contact the Bacillus Genetic Stock Center at the Department of Microbiology at The Ohio State University. Academic and non-profit users are asked to make a voluntary payment of $35 per culture or may subscribe to a yearly service plan for $195. No academic scientist will be turned away, even if he or she lacks funds.
It is very simple to get a pure spore crop in the lab itself. If you don't want to order it then just take the B. subtilis strain that is available in your lab and then use the protocol mentioned in the following link. It's a kind of a self advertisement, I know, but nevertheless it will serve the purpose.