I have to germinate the seeds of Alnus nepalensis and Cinnamomum burmanii (阴香）. I already carried out pre-germination test, but failed. In alder seeds, Ca (OH)2 was used and no one was germinated even after 30days. And i don't know how to germinate Cinnamomum burmanii. Can you let me know pre-treatment method of these seeds and what is important for germination for two seeds? If possible, let me know the information about this. Chinese literature is also possible.
Cinnamomum burmannii seed germination:
See the info provided from the link, probably you need to use fresh seeds to improve their germination efficiency.
The seed has a short viability and is best sown as soon as it is ripe in a shaded nursery bed. Only fresh seeds germinate reliably. Remove the fruit pulp since this can inhibit germination. Germination can take 1 - 6 months at 20°C. Fresh seed germinates in 5-15 days.
http://tropical.theferns.info/viewtropical.php?id=Cinnamomum+burmannii
Seed viability is a problem - check whether the seed lot with you is viable (TTC test) - removal of pulp as suggested is recommended for Cinnamomum burmanii. Scarification treatment also should have a promoting effect.
http://search.informit.com.au/documentSummary;dn=372995800260668;res=IELHSS
http://tao.wordpedia.com/show_pdf.ashx?sess=i3utcc2y35eutk45et32pt55&file_name=JO00000874_45-1_55-66&file_type=r
Check seed viability for Alnus also - try germinating at low temp.
http://www.indianforester.co.in/index.php/indianforester/article/view/8608
In case of Alnus seeds try use a x-ray test (if you can) and check develop of embryo. I don't have any experience with Cinnamomum burmannii seeds.
Regarding Alnus nepalensis and judging from the european alder (Alnus glutinosa) I would suggest:
1. Using light (white light) for at least 12 h daily,
2. Check warm temperatures, ie 20-30 C.
3. Use cut-test (try on a sample at the beginning) and correct each sample after germination is completed - the genus tends to make lots of empty seeds.
4. If, after all 3 above do not render a germination percentage higher than 80%, you could try cold stratification (maybe 1-3 months) and transfer afterwards to a warm temperature.
Good luck!
PS Have a look at https://www.researchgate.net/publication/262600509_Sexual_reproduction_in_native_trees_of_Greece
Conference Paper Sexual reproduction in native trees of Greece
I have no idea about the seedstructure of Alnus. You need to try different techniquess
such treatment of cold temp, for some time, treatment with conc. sulfuric acid for few minutes, then wash thoroughly, stratication, scarification, treatment with high temp. etc. It is not possible to suggest technique without knowing the seed structure.
you have to check the seed structure, seed coat type. if iys hard then you have to use the physical treatments and chemical treatments. in physical methods if you dont get any successful germination then try its combination with some chemicals like HCl, H2SO4, HNO3, gibberelic acid etc. or have combination of urea, KNO3, thiophosphate in different combinations. if you need details for the same let me give you some methods which helped me in giving good results in seed germination study.
In case of Alnus napelensis Stratification is advisable and also raise the seeds in bed.
In case of Cinnamomum burmannii washing pulp and scarifying seed coat and treat with low concentration (0.1 - 0.3 N) HCl for 30 - 60 min is advisable,'
All the best
Perhaps the Alnus nepaulensis seed may have the hard seed coat. Hence it may tried through scarification, hot water treatment, chemical treatment like GA3 + thiourea alone and in combination as well as treating the seeds with low conc. of HCL/ H2SO4. Moreover u please try freshly harvested seeds soaking in water for different times interval period. rying these may help in breaking the hard seed coat as we;; as dormancy if any. thanks.
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