What parameters of quality control would I have to consider for antimicrobial toothpaste?

Hello, my name is Bianca and as a project for university I have assigned the manufacturing of antimicrobial toothpaste- quality control department. I tried to research online but I could not find...

17 January 2021 918 3 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

Would anyone have an explanation of the occurrence of this phenomenon in the LS-Dyna simulation in the drum seaming roll process?

Hello, I am simulating in Ls-Dyna a drum seaming process that consists of closing a drum, ie the lid with the body using two additional tools (figure 1). The tools are set as rigid shells and the...

26 August 2019 4,808 1 View

How to write a methodological paper?

Dear all, I would like to write a methodological paper. Notably, I would like to explain how a particular methodology can be used to study problems related to a specific field of studies (i.e....

21 October 2018 2,254 22 View

Could you suggest must-read literature of socio-ecological systems?

Hi everyone, I might be approaching the field of socio-ecological systems to write the final paper of my PhD. I would like to get an idea of a super MUST-READ literature to get an overview of the...

04 April 2018 7,009 26 View

Has anyone one used (snap)frozen tissue samples in a ProcartaPlex Multiplex Immunoassay?

The guidelines states that it is suitable for serum, plasma and cell cultured lysates. Theoretically can you use animal tissue and make a tissue lysate?

21 September 2017 8,408 2 View

Regarding the use of water samples, what other testing methods can be used besides Coliform test for water that are more efficient and cost-friendly?

19 April 2017 5,018 3 View

Missing concentration-dependend effect of IL-2 concentration by TERB and NAD on human PBMC?

I have some questions regarding the results of my experiments. I need inspiration and ideas to discuss these results. Hopefully someone can help me to explain this. A short introduction of my...

21 January 2017 8,818 3 View

Is anyone aware of a described gene sequence for feline Angiotensin II?

I am trying to design an RT-qPCR primer for Angiotensin II in cats.

15 August 2016 3,872 3 View

Ways to produce aluminum powder from aluminum bar?

I am looking for ways to produce powder from an aluminum bar. I can't find an available grinder/mill, so I am looking for an alternative. I saw a method wherein a steel plate and a steel bar were...

07 June 2016 3,949 15 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View