I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method or protocol to find out these genes?
Interesting subject! Which species of amphibians affected by fungus are in Your attention?
http://www.sciencemag.org/content/332/6033/1097.full.html
Good paper to read on the topic. They used random transposon mutagenesis. I study amphibian disease and would love to hear what you find!
Maybe start with this paper
"A molecular perspective: biology of the emerging pathogen Batrachochytrium dendrobatidis"
By: Rosenblum, Erica Bree; Fisher, Matthew C.; James, Timothy Y.; et al.
DISEASES OF AQUATIC ORGANISMS Volume: 92 Issue: 2-3 Pages: 131-147 Published: NOV 25 2010
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