Which Kit is better to do Chromatin Immunoprecipitation (ChiP) with a protein tagged with His-Tag in Cyanobacteria?

I have expressed a transcriptional factor in the Cyanobacterial cell. The target protein is tagged with His-Tag. I would like to do Chip assay (using qRT-PCR). Which Kit is better for this assay?...

31 December 2014 5,944 1 View

Which solvent should be used to make 10 mg/mL Tetrahydrofuran for GC-MS analysis of organic acids?

I would like to do GC-MS analysis for metabolic profiling. In the protocol, it is said the standard such as Decanoic acids should be dissolved in 10 mg/mL Tetrahydrofuran. I have liquid...

11 December 2014 8,675 2 View

How can one solve Arcing during Electroporation of Cyanobacterial cell?

09 October 2014 8,739 9 View

What antifungal can I use in the plate for tissue culture of agrobacterium mediated transform rice callus?

08 September 2014 10,000 3 View

Why is there a different melt curve for the same primer in two different samples from two separate conditions of a bacteria?

I am running qRT-PCR for quantification of gene expression in Cyanobacteria. The designed primer is showing single melt curve at Wild type's sample but it is showing multiple curves in sample from...

06 July 2014 6,609 5 View

How can I remove RNA from isolated genomic DNA?

I have isolated DNA from animal tissue. The concentration of DNA has been measured as 200ng/microL. I am afraid that the genomic DNA might have contamination with RNA. Now, If do like to remove...

02 March 2014 3,982 15 View

Can anyone explain a smear band above the target band in Western Blotting?

I am doing western blotting of a protein tag with Anti-Myc. It seems that it has some unspecific, smear band both up side and lower side of the target band (please see image below). Can anyone...

02 March 2014 5,879 5 View

Is the Nanodrop machine reliable enough to measure OD730 for cell counting?

I am using a Nanodrop machine to measure cell density at 730 ( Cyanobacterial cell). But I have some doubt that sometimes this machine gives varied data in replication. Does anybody have...

02 March 2014 7,602 6 View

How can I determine the contamination of DNA in isolated RNA?

I have isolated RNA from bacterial cells. But I want to be sure that there is no contamination with DNA. How can I be sure? Which experiment is suitable for that?

02 March 2014 188 15 View

What are the causes of dissimilar differential expression of genes in microarray and qRT-PCR? Which one is most reliable?

I have done microarray to check differential expression in a given condition. I got some genes have changed differentially upto 10 folds. However, when I checked it by qRT-PCR, I found there is no...

02 March 2014 7,927 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View