18 Questions 17 Answers 0 Followers
Questions related from Md. Rezaul Islam Khan
I have expressed a transcriptional factor in the Cyanobacterial cell. The target protein is tagged with His-Tag. I would like to do Chip assay (using qRT-PCR). Which Kit is better for this assay?...
01 January 2015 6,009 1 View
I would like to do GC-MS analysis for metabolic profiling. In the protocol, it is said the standard such as Decanoic acids should be dissolved in 10 mg/mL Tetrahydrofuran. I have liquid...
12 December 2014 8,743 2 View
I am trying to Electroporation of Synechosystis cells using 2.5 Kv ( 12.5 KV/ Cm) voltage in 2mM, 1 mM HEPES (pH 7.5)or 5 mM NaCl. I washed the cells three times before set for Electroporation...
10 October 2014 8,847 9 View
I have transformed rice plant by Agrobacterium mediated gene transfer system but getting problem of contamination by fungus. Is there any problem if I use antifungal in that plate to get rid of...
09 September 2014 10,089 3 View
I am running qRT-PCR for quantification of gene expression in Cyanobacteria. The designed primer is showing single melt curve at Wild type's sample but it is showing multiple curves in sample from...
07 July 2014 6,764 5 View
I have isolated DNA from animal tissue. The concentration of DNA has been measured as 200ng/microL. I am afraid that the genomic DNA might have contamination with RNA. Now, If do like to remove...
03 March 2014 4,055 15 View
I am doing western blotting of a protein tag with Anti-Myc. It seems that it has some unspecific, smear band both up side and lower side of the target band (please see image below). Can anyone...
03 March 2014 5,993 5 View
I have isolated RNA from bacterial cells. But I want to be sure that there is no contamination with DNA. How can I be sure? Which experiment is suitable for that?
03 March 2014 260 15 View
I am using a Nanodrop machine to measure cell density at 730 ( Cyanobacterial cell). But I have some doubt that sometimes this machine gives varied data in replication. Does anybody have...
03 March 2014 7,671 6 View
I have done microarray to check differential expression in a given condition. I got some genes have changed differentially upto 10 folds. However, when I checked it by qRT-PCR, I found there is no...
03 March 2014 8,041 5 View
I have some data to make cluster analysis. Suppose these data are five columns; ID of gene, t-value, P.value, Adj.P.value and FC. I would like to cluster it and find a group of data showing...
02 February 2014 3,055 3 View
I have isolated RNA from a Cyanobacterial culture using TRIZOL. The concentration of RNA is around 500ng/microL, and purification parameter as well as gel run indicating good enough the quality...
04 April 2013 3,572 15 View
FLP, a gene from Saccharomysis, is widely used in FLP/FRT recombination system. As it is a gene from Eukaryotic cells, it has an intron. I would like to express this gene in a prokaryotic cell....
04 April 2013 6,083 1 View
Robotyping is now very popular to characterize the species of bacteria. I do not have a clear idea of how this technique help to characterize microbes. I need some suggestions.
03 March 2013 399 3 View
I would like to design and select primers for qRT-PCR of genes from Cyanobacteria which is Prokaryotic organism. I need to know which parameters are crucial to select the best primers. I would...
03 March 2013 5,841 2 View
I want to check the change of metabolites in Cyanobacteria after supplementing glucose in the media. I am interested to see the metabolites of Glycolytic, TCA cycle and OPP pathways. Which methods...
03 March 2013 1,481 3 View
I want to measure photaxicity properties of a Cyanobacteria. Some scientists have used 0.5% agar in BG-11 media and unidirectional light to measure the ability of Cyanobacteria to move toward...
03 March 2013 7,070 4 View
The scientific articles are published with the purpose of telling the other scientists of the same and other fields about the progress and new advancement of the field. But , most of the time...
01 January 1970 1,893 4 View
