How can i find detailed western blotting protocol for nucleoplasm's proteins?

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Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

How to purify an S-tagged recombinant protein?

Guys, I have a pET vector 30a+ and my gene of interest has been inserted between XhoI and KpnI sites. Nearest to it is only an S-tag present. How can I purify my protein and remove the s-tag from...

17 December 2020 9,718 3 View

Flight direction/pattern of moth?

I would like to determine the movement of moths in response to mating disruption pheromone traps. Pheromone lures (matting disruption) were placed in the center of the treatment plot. High...

23 September 2020 6,293 4 View

Is there an epidemiological prediction model to provide a timeline when COVID-19 pandemic would end?

Although in electronic media many predictions about COVID-19 abound, is there a scientific prediction when the this pandemic would die down? Would this time come when it has affected 50-70% of the...

27 March 2020 3,069 7 View

How is the comparative expression pattern of apolipoproteins in the hepatocytes?

Hi everyone; I need data on comparison of apolipoproteins expression in the normal liver. I want to know which type of the apolipoproteins is highly expressed than others in the normal...

15 March 2020 605 3 View

How to run GUI applications inside Docker Containers?

Actually I developed a gui app using tkinter(Python). But can't display tkinter gui from within docker. Did someone has this problem before ? How can I fix it ? Please suggest me some useful links.

05 March 2020 5,457 3 View

Where can I find template of "safety science" journal?

I have ransacked the web and am unable to find any word or latex template of "safety science" journal. Can anyone suggest the template?

30 January 2020 5,342 2 View

How can I get the quasi-periodic and periodic orbits from the Poincare surface of section in the restricted three body problem?

By plotting surface of section, we get many island like surfaces.

26 August 2019 836 1 View

Can anyone please guide me how to calculate the separate quantities of Mg(OH)2, KOH and NaOH in the mortar samples?

I have to calculate the quantity of Mg(OH)2, KOH and NaOH separately in the mortar samples. Similarly as we calculate percentage mass loss of Ca(OH)2 by TGA testing of mortar in the temperature...

22 July 2019 2,439 3 View

Defiing parameters for Materials in Comsol Multiphysics?

I have to introduce Si(1-x)Cx as source drain material in 110 direction. Needed parameters are elasticity matrix, density, lattice constant and initial strain values. A document on formulas or...

02 July 2019 2,453 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Cyrille Girard

Try to google it...or?

Bhairavi Vajaria

http://www.aun.edu.eg/molecular_biology/Protein%20workshop/WB-beginner.pdf

Md. Golzar Hossain

Lysis buffer and primary Ab against your target protein are important. You can find detail of WB protocol in different company's HP....

https://www.cusabio.com/m-244.html#a01

Article Western Blotting: Sample Preparation to Detection

https://www.abcam.com/protocols/general-western-blot-protocol

Andreas Eisenreich

Sure, google is helpful, such a sNCBI tools, too.

A potentially helpful contribution is: Nat Protoc. 2016 Mar;11(3):553-65. doi: 10.1038/nprot.2016.032.

Yousuf Elhaei

hi i thanks all you who answered my question but i want to know how can i optimize WB protocol for the proteins;for example what percent of resolving gel i should to use?