Hi!

I have a bacterial strain that changes the pH of the broth making it acidic. I want to get a knock-out mutant that is unable to lower the pH.

Since I'm using transposon mutagenesis without finding the transformant I want, I was considering to analyse the annotated reference genome to find the genes involved.

Honestly, I don't know where to start from.

I mean: should I look into the methabolic pathway using Kegg database or there are better ways to dig into the genome? Shoul I look for genes involved in the production of acidic compunds? How?

Any advice is appreciated.

Thanks!