What is the best ITS primers for discrimination of fungi species?

Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by a few limitations, the ITS will remain as the key choice for fungal identification. I usually see the ITS 1F...

11 August 2020 3,597 10 View

How can I prepare Lysozyme digestion buffer for Invitrogen DNA extraction kit (Gram positive bacteria)?

I'd like to prepare a Lysozyme and Lysozyme Digestion Buffer (25mM Tris-HCL, pH 8.0, 2.5mM EDTA, 1% Triton X-100) for bacterial cell lysate with a final Lysozyme concentration of 20mg/mL. I need...

29 July 2020 2,818 2 View

What kind of drug molecules could be fused to produce fused prodrugs?

There are some studies on fused drug molecules. Sultamicillin is one of the example for this type of prodrugs. The drug would be hydrolized and reveals two different active molecules which is...

18 April 2020 4,758 3 View

How bin size affects Particle Size Distribution?

Hi, I have question which I could not find an appropriate answer. By default, each laser diffraction instrument plots PSD using different bin sizes on the x-axis. This means, using default...

21 October 2019 6,899 3 View

Any guidelines for number of cores/problem size in parallel CFD simulations?

Hello, I constantly submit parallel jobs in a shared HPC cluster for my OpenFOAM flow simulations and like everyone else, I have to decide how many cores I need. Our cluster runs constantly over...

11 May 2019 8,329 6 View

Simulation and measurement results are different. What can be the reason?

I simulated a patch antenna. I saw that the best S11 value is at 5.9GHz but when I measured I saw that it works at 5.93 GHz. What can be the reason?

23 September 2017 9,104 2 View

How can I use PCR without genomic extraction on N2a cells?

I have primers and i think i have to lysis the N2a cells but after that i couldn't find any protocol like how much i must add for template or other things.

06 June 2017 8,442 3 View

How can we draw or get the centerline of a surface using Solidworks?

I would like to learn drawing or getting centerline of a surface in Solidworks. I search on internet but I found limited information. I found a programs like VMTKLab.There is a section for...

12 May 2015 2,748 7 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View