I have kits and stuff but I also want to try with the basic protocol. I found some different things like with phenol,chloroform or the other things but i am not sure if it can work against the N2a cells.
There are many difficulties in farmers' access to the market in the agricultural sector, especially in underdeveloped and developing countries. Therefore, how digital marketing channels and...
25 December 2023 1,569 0 View
I've downloaded the new "Mendeley Reference Manager for Desktop" software after the support for "Mendeley Desktop" (latest version 1.19.8) has been dropped. Now I can't find the "Update Details"...
03 December 2023 3,255 1 View
Hello everyone, for the Türksat satellite competition, I need to save the live image I received from the camera to the SD card and send it live to the ground station. How can I do this? and I need...
24 September 2023 6,596 0 View
Is it better to use MUSCLE or CLUSTALW to align nucleotide sequences of genes belonging to the same genus?
02 June 2022 2,028 3 View
Hi I have a question for you. I use snapgene viewer to see and work the genes, however each time when i need to work a specific exon or intron, i enter this dna seq by hand. I am looking for an...
01 June 2022 8,717 0 View
Hi, I have been looking at the La Haute Borne wind farm data for my research. The data descriptions are given...
17 February 2022 5,724 1 View
All of the peptides (Hydrophobic, hydrophilic and neutral) give very strong peaks between 2-5 minutes in analytical HPLC, regardless of their properties. Even though I reversed the method, the...
12 October 2021 2,772 4 View
Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by a few limitations, the ITS will remain as the key choice for fungal identification. I usually see the ITS 1F...
11 August 2020 3,880 10 View
I'd like to prepare a Lysozyme and Lysozyme Digestion Buffer (25mM Tris-HCL, pH 8.0, 2.5mM EDTA, 1% Triton X-100) for bacterial cell lysate with a final Lysozyme concentration of 20mg/mL. I need...
29 July 2020 3,024 2 View
Description A quinolone antibiotic is a member of a large group of broad-spectrum bacteriocidals that share a bicyclic core structure related to the substance 4-quinolone. The medications are gone...
26 April 2020 2,184 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View