How can I extract DNA from yeast cells?

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Nadine A. Gund

HI, there are a lot of kits for DNA extraction from yeast cells. Ask the companies for free samples.

http://www.epibio.com/applications/nucleic-acid-purification-extraction-kits/dna-purification-genomic/masterpure-yeast-dna-purification-kit

http://www.piercenet.com/product/yeast-dna-extraction-kit

http://www.zymoresearch.com/yeast

....

Cheers, Nadine

Dominique Liger

Hi there,

For what purpose do you need this DNA? If it's just to use it as a template for PCR, you don't need a lot.

Carlo A. Klein

Hi, i can recommend this page for all kind of methods working with yeast. For standard protocols you should follow the first two links on the page. http://wiki.yeastgenome.org/index.php/Methods

Have fun!

William D Bradford

If you are just doing endpoint PCR you can heat the cells in 0.02 M NaOH for 10 minutes.

For qPCR we wash with water, treat with zymolyase, then incubate at 95 C for 5 minutes to lyse. The lysates go directly into the qPCR mastermix.

Uma Kranthi

Thank you ... I ll give it a try

Sandra Mariotto

Do use a specific kit. Is the best and easy.

Sandip Chakraborty

You can please refer to the links below to obtain certain valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182553/

2. http://www.molecularcloning.com/index.php?prt=16

3. http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200020

4. https://www.thermofisher.com/order/catalog/product/78870